Recovering protein conformations from single-particle cryo-EM data via indirect shape matching gradient flows

Single-particle cryo-electron microscopy images a macromolecule as many noisy tomographic projections of its electrostatic potential.

Single-particle cryo-electron microscopy images a macromolecule as many noisy tomographic projections of its electrostatic potential. We reconstruct the protein backbone directly from such projections, as an atomic point cloud, without the intermediate step of reconstructing the…