Structural mechanism governing the directionality of bridge recombination.

Bridge recombinases from the IS110 family of transposons, such as IS621, associate with a bridge RNA (bRNA) to mediate programmable recombination between donor DNA and target DNA 1,2 .

Bridge recombinases from the IS110 family of transposons, such as IS621, associate with a bridge RNA (bRNA) to mediate programmable recombination between donor DNA and target DNA 1,2 . Although insertion is mediated by the recombinase-bRNA complex, it remains unknown how IS621…