Omega-seq: ultra-low-background RNA sequencing with faithful molecular counting and precise transcript-end capture

PCR-based RNA sequencing methods generate phantom unique molecular identifiers (UMIs) when residual UMI-bearing oligonucleotides reprime during amplification, inflating molecule counts and cor- rupting relative abundances.

PCR-based RNA sequencing methods generate phantom unique molecular identifiers (UMIs) when residual UMI-bearing oligonucleotides reprime during amplification, inflating molecule counts and cor- rupting relative abundances. They also incur non-specific amplification that limits…