Bacteriophage genome-wide transposon mutagenesis.

Bacteriophage genomes are densely packed with coding sequences and frequently encode genes of unknown function.

Bacteriophage genomes are densely packed with coding sequences and frequently encode genes of unknown function. Unbiased phage functional genomics approaches are therefore needed, particularly for large lytic phages. Here, we harness the mariner transposase to develop phage…