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Science & ResearchJul 16, 2026

Extraction workflow determines marker-specific recovery andreproducibility in leaf-litter eDNA metabarcoding

Forest-floor leaf litter is a dynamic and structurally complex ecological transition zone and thus a promising substrate for terrestrial eDNA metabarcoding.

Forest-floor leaf litter is a dynamic and structurally complex ecological transition zone and thus a promising substrate for terrestrial eDNA metabarcoding. Yet, extraction workflows for this heterogeneous matrix remain poorly standardized, especially in tropical systems, making…

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