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Science & ResearchAug 22, 2026

Scarless conditional sgRNAs via endogenous mascRNA processing enable rapid and temporally controlled genome editing

Precise temporal control of gene editing is essential for studying dynamic biological processes, interrogating essential gene function, and improving the interpretability of pooled perturbation screens.

Precise temporal control of gene editing is essential for studying dynamic biological processes, interrogating essential gene function, and improving the interpretability of pooled perturbation screens. Cre-dependent single guide RNA (sgRNA) switches provide temporal regulation…

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