Prompting Beyond Pairs: Decoupled Semantic Supervision for Knowledge-Guided Multiplex Virtual Staining

Virtual staining provides a non-invasive alternative to fluorescence microscopy, yet existing deep learning approaches fundamentally rely on pixel-aligned, multiplexed fluorescence targets for supervision.

Virtual staining provides a non-invasive alternative to fluorescence microscopy, yet existing deep learning approaches fundamentally rely on pixel-aligned, multiplexed fluorescence targets for supervision. This dependence on rigidly paired data limits scalability, constrains…