Allosteric Constraints on Rewiring Inducible Repressors

Precise chemical control of transgene expression is central to synthetic biology, mammalian cell engineering, and gene therapy.

Precise chemical control of transgene expression is central to synthetic biology, mammalian cell engineering, and gene therapy. Although tetracycline-responsive systems are widely used, converting an inducible repressor into a robust co-repressible regulator remains difficult.…